Journal: Heliyon
Article Title: Hepatocellular carcinoma patients serum modulates the regenerative capacities of adipose mesenchymal stromal cells
doi: 10.1016/j.heliyon.2024.e24794
Figure Lengend Snippet: Effect of HCC serum treatment for 6 days on the migration potential and cancer progression of hA-MSCs and Huh-7 cells. (A, G) Normalized gene expression analysis using real-time PCR in normal and HCC serum-treated hA-MSCs and Huh-7 respectively. (B, H) Protein expression of CCL2, IL8, and TIMP-1 assessed by Enzyme-linked immunosorbent assay (ELISA) in normal and HCC serum-treated hA-MSCs and Huh-7respectively. (C, E) Scratch assay for normal and HCC serum-treated hA-MSCs and Huh-7 respectively. This was assessed at zero time, 24 h, and 48 h. Pictures were taken using an inverted fluorescence microscope and scale bars (200 μm) were added. (D, F) Bar chart representation of the percentage of closure of the scratch in normal and HCC serum-treated hA-MSCs and Huh-7 respectively.
Article Snippet: A transmission electron microscope (TEM, Philips EM 208S, Tokyo, Japan) was used to examine and image cells at an acceleration voltage of 80 kV.
Techniques: Migration, Gene Expression, Real-time Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay, Wound Healing Assay, Fluorescence, Microscopy